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The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci

Autor: Erich Willems Mi, 19.04.2023 - 14:46;
TitelThe giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci
MedientypJournal Article
Jahr der Veröffentlichung2021
AutorenSchloissnig, S., A. Kawaguchi, S. Nowoshilow, F. Falcon, L. Otsuki, P. Tardivo, N. Timoshevskaya, M. C. Keinath, J. J. Smith, R. S. Voss, and E. M. Tanaka
JournalProceedings of the National Academy of SciencesProceedings of the National Academy of Sciences
Volume118
Problem15
Seitennummerierunge2017176118
Veröffentlichungsdatum2021
Zusammenfassung

Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration.Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration.
URLhttps://uknowledge.uky.edu/biology_facpub/207/?utm_source=uknowledge.uky.edu%2Fbiology_facpub%2F207&utm_medium=PDF&utm_campaign=PDFCoverPages
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