'Es spielt keine Rolle, wie schön deine Theorie ist. Es spielt keine Rolle, wie klug du bist. Passt sie nicht zu den experimentellen Ergebnissen/Beobachtungen, dann ist sie falsch!'. {nach Richard Feynman, Nobelpreis für Physik 1965}
Titel | The giant axolotl genome uncovers the evolution, scaling, and transcriptional control of complex gene loci |
Medientyp | Journal Article |
Jahr der Veröffentlichung | 2021 |
Autoren | Schloissnig, S., A. Kawaguchi, S. Nowoshilow, F. Falcon, L. Otsuki, P. Tardivo, N. Timoshevskaya, M. C. Keinath, J. J. Smith, R. S. Voss, and E. M. Tanaka |
Journal | Proceedings of the National Academy of SciencesProceedings of the National Academy of Sciences |
Volume | 118 |
Problem | 15 |
Seitennummerierung | e2017176118 |
Veröffentlichungsdatum | 2021 |
Zusammenfassung | Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration.Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth Factor 8 (Axfgf8) region. The axolotl serves as a primary model for studying successful regeneration. |
URL | https://uknowledge.uky.edu/biology_facpub/207/?utm_source=uknowledge.uky.edu%2Fbiology_facpub%2F207&utm_medium=PDF&utm_campaign=PDFCoverPages |
'Es spielt keine Rolle, wie schön deine Theorie ist. Es spielt keine Rolle, wie klug du bist. Passt sie nicht zu den experimentellen Ergebnissen/Beobachtungen, dann ist sie falsch!'. {nach Richard Feynman, Nobelpreis für Physik 1965}